Notice: Undefined offset: 1 in /website/encore_alpha/gene/index.php on line 599
CRKL RNA-Seq in K562



CRKL      CRISPR in K562      Control:

General Information
RBPCRKL
Cell_LineK562
MethodCRISPR
Exp_NameCRKL-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1CRKL-BGKcLV42-19
Rep2CRKL-BGKcLV42-20
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR42.2
Rep2_qPCR44.4
Rep1_WB87.2
Rep2_WB84.8
Antibody Cat#38710S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1533




Experiment Information (Status: Sequenced)
BGKcLV42-19BGKcLV42-20
idx00
TRCN#_or_BGC#BGC#0001121BGC#0001121
shRNA_or_gRNA_sequenceGTCCGAGGAGTCGAACCTGGGTCCGAGGAGTCGAACCTGG
PAMCGGCGG
NameCRKL_91CRKL_91
Sample_IDBGKcLV42-19BGKcLV42-20
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameCRKLCRKL
qPCR_result42.244.4
Ave_qPCR43.3
RT-qPCR_primer-Ftaagatcggggaccaggaat
RT-qPCR_primer-Rggttgggtgctgagacagat
protein_conc20762373
WB_result87.284.8
Ave_WB86.0
WB_DONE_date5/6/24
MW39KDTUBULIN
IP
antibody_Cat#38710Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID57775778




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CRKLProduct_ID: 38710S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: CRKL-human		 CRKL-HEPG2-CRISPR-38710S.png<br>Caption: Western blot following CRISPR against CRKL in HepG2 whole cell lysate using CRKL specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CRKL. CRKL protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_38710S_1_CRKL.png<br>Caption: IP-WB analysis of 38710S whole cell lysate using the CRKL specific antibody, 38710S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CRKL-specific antibody, 38710S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CRKL-K562-CRISPR-38710S.png<br>Caption: Western blot following CRISPR against CRKL in K562 whole cell lysate using CRKL specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CRKL. CRKL protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-19BGKcLV42-20
Sample_IDBGKcLV42-19BGKcLV42-20
Sample Name
Sample NameCRKL-BGKcLV42-19CRKL-BGKcLV42-20
RBPCRKLCRKL
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1306013061




Library-Prep Information
BGKcLV42-19BGKcLV42-20
Sample #1718
Sample NameCRKL-BGKcLV42-19CRKL-BGKcLV42-20
Sample_Name_Alias
Index Well PositionA03B03
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp297292
Peak_Molarity83.1099.30
libSampleQC_DNA_WellDNA_Library_set73/set1/A3.pngDNA_Library_set73/set1/B3.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/A3.pngRNA_Library_set73/set1/B3.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-19BGKcLV42-20
RBPCRKLCRKL
Batch_IDBGKcLV42BGKcLV42
WB_result87.20084.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42864287




Sequencing Information
BGKcLV42-19BGKcLV42-20
Sample_IDBGKcLV42-19BGKcLV42-20
Sample NameCRKL-BGKcLV42-19CRKL-BGKcLV42-20
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads95,563,63630,845,781
total_aligned_reads81,623,05926,578,344
unique_aligned_reads74,719,28524,418,449
percent_uniqueAligned0.781880.79163
correlation_replicates0.9956040.995604
spikein_reads12,028962
percent_spikeins0.000130.00003
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31533154




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database