SMYD3      CRISPR in K562      Control: NT-BGKcLV19

General Information
RBPSMYD3
Cell_LineK562
MethodCRISPR
Exp_NameSMYD3-BGKcLV19-K562
ENCODE_series_IDENCSR441UTQ
Batch_IDBGKcLV19
Pool IDPool-200928
Local_Set_Nameset45_K
ENCODE_KD_Exp_IDENCSR775USN
ENCODE_CN_Exp_IDENCSR978MMZ
Rep1SMYD3-BGKcLV19-51
Rep2SMYD3-BGKcLV19-52
CN1NT-BGKcLV19-1
CN2NT-BGKcLV19-2
Rep1_qPCR31.23
Rep2_qPCR45.08
Rep1_WB91.4
Rep2_WB90.6
Antibody Cat#PA5-31919
Antibody Lot#Lot# UA2701668D
Antibody DCC IDENCAB101UYP
StatusReleased
ProjectENCODE4
ID60




Experiment Information (Status: NotSatisfied)
BGKcLV19-49BGKcLV19-50
idx723724
TRCN#_or_BGC#BGC#0000429BGC#0000429
shRNA_or_gRNA_sequenceGCAGTCCGAGACATCGAGGTGCAGTCCGAGACATCGAGGT
PAMGGGGGG
NameSMYD3_89SMYD3_89
Sample_IDBGKcLV19-49BGKcLV19-50
transduction_Date11/6/1911/6/19
daysDay 6Day 6
RBP_nameSMYD3SMYD3
qPCR_result86.2982.47
Ave_qPCR
RT-qPCR_primer-FAGACATCGAGGTGGGAGAGG
RT-qPCR_primer-RGGCAACGGAAACAGTCACAT
protein_conc33873541
WB_result88.789.2
Ave_WB89.0
WB_DONE_date12/5/19
MW49kd
IPRabbit / I
antibody_Cat#PA5-31919
Antibody DCC IDno ID
submitted_to_DCC_date
Rep1_TPM101.80
Rep2_TPM103.90
Action
Library_start_date
repeat_library
Note
ID48434844




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SMYD3Product_ID: PA5-31919
Lot_ID: UA2701668D
Source: Thermo Fisher
Target Name: SMYD3-human		 smyd3-HEPG2-CRISPR-PA5-31919.png<br>Caption: Western blot following CRISPR against SMYD3 in HepG2 whole cell lysate using SMYD3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMYD3. SMYD3 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
 ThermoFisher_PA5-31919_UA2701668D_SMYD3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the NUP35 specific antibody, A301-781A. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the NUP35-specific antibody, A301-781A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SMYD3-K562-CRISPR-PA5-31919.png<br>Caption: Western blot following CRISPR against SMYD3 in K562 whole cell lysate using SMYD3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMYD3. SMYD3 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGKcLV19-49BGKcLV19-50
Sample_IDBGKcLV19-49BGKcLV19-50
Sample Name
Sample Name
RBPSMYD3SMYD3
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2020-09-172020-09-17
ProjectENCODE4ENCODE4
Note
ID57495750




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database