MBNL1      shRNA in K562      Batch: BGKLV29

Experiment Information (Status: NotSatisfied)
BGKLV29-43BGKLV29-44
idx578579
TRCN#_or_BGC#TRCN0000063967TRCN0000063967
shRNA_or_gRNA_sequenceCCCATAATATCTGCCGAACATCCCATAATATCTGCCGAACAT
PAM
NameMBNL1MBNL1
Sample_IDBGKLV29-43BGKLV29-44
transduction_Date8/11/20158/11/2015
days
RBP_nameMBNL1MBNL1
qPCR_result7.841.0
Ave_qPCR24.4
RT-qPCR_primer-Fctgccgaacatctgactagc
RT-qPCR_primer-Ratggctaatgacctcgtcct
protein_conc30853487
WB_result
Ave_WB
WB_DONE_date
MW
IP11
antibody_Cat#GTX112624GTX112624
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM14.03904220
Rep2_TPM11.24537820
Action
Library_start_date
repeat_library
Note
ID657658




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
MBNL1Product_ID: GTX112624
Lot_ID: 40562
Source: GeneTex
Target Name: MBNL1-human
MBNL1-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against MBNL1 in K562 whole cell lysate using MBNL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against MBNL1.MBNL1 protein appears as the green band, Tubulin serves as a control and appears in red.




Experiment Status
BGKLV29-43BGKLV29-44
Sample_IDBGKLV29-43BGKLV29-44
Sample Name
Sample Name
RBPMBNL1MBNL1
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2019-06-262019-06-26
ProjectENCODE3ENCODE3
Note
ID15771578




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database