RPL8 RNA-Seq in HepG2

General Information


RBP	RPL8
Cell_Line	HepG2
Method	CRISPR
Exp_Name	RPL8-BGHcLV52-HepG2
ENCODE_series_ID
Batch_ID	BGHcLV52
Pool ID	Pool-260528
Local_Set_Name	set82
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1	RPL8-BGHcLV52-45
Rep2	RPL8-BGHcLV52-46
CN1	NT-BGHcLV52-1
CN2	NT-BGHcLV52-2
Rep1_qPCR	66.3
Rep2_qPCR	63.2
Rep1_WB	63.7
Rep2_WB	50.8
Antibody Cat#	A305-059A
Antibody Lot#	LOT #1
Antibody DCC ID
Status	Submitted
Project	ENCORE2
ID	1727

Experiment Information (Status: Sequenced)

	BGHcLV52-45	BGHcLV52-46
idx	1993	1994
TRCN#_or_BGC#	BGC#0001261	BGC#0001261
shRNA_or_gRNA_sequence	GAGCTGTTCATTGCCGCCGA	GAGCTGTTCATTGCCGCCGA
PAM	GGG	GGG
Name	RPL8_93B	RPL8_93B
Sample_ID	BGHcLV52-45	BGHcLV52-46
transduction_Date	1/8/26	1/8/26
days	D6	D6
RBP_name	RPL8	RPL8
qPCR_result	66.3	63.2
Ave_qPCR	64.7
RT-qPCR_primer-F	ttgtgtattgcggcaagaag
RT-qPCR_primer-R	gattgtaccctcaggcatgg
protein_conc	2131	1866
WB_result	63.7	50.8
Ave_WB
WB_DONE_date	2/9/26
MW	28KD
IP
antibody_Cat#	A305-059A	LOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	10182	10183

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
RPL8	Product_ID: A305-059A Lot_ID: 1 Source: Bethyl Labs Target Name: RPL8-human

Experiment Status

	BGHcLV52-45	BGHcLV52-46
Sample_ID	BGHcLV52-45	BGHcLV52-46
Sample Name
Sample Name	RPL8-BGHcLV52-45	RPL8-BGHcLV52-46
RBP	RPL8	RPL8
Cell_Line	HepG2	HepG2
Exp UID
Status	Sequenced	Sequenced
Status_date	2026-09-16	2026-09-16
Project	ENCORE2	ENCORE2
Note
ID	14694	14695

Library-Prep
Sequencing

Library-Prep Information

	BGHcLV52-45	BGHcLV52-46
Sample #	27	28
Sample Name	RPL8-BGHcLV52-45	RPL8-BGHcLV52-46
Sample_Name_Alias
Index Well Position	C04	D04
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2026-05-18	2026-05-18
Lib_ID	Lib-260518	Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp	313	311
Peak_Molarity	55.40	64.10
libSampleQC_DNA_Well	DNA_Library_set82/set1/C11.png	DNA_Library_set82/set1/D11.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set82/set1/C4.png	RNA_Library_set82/set1/D4.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2026-05-21	2026-05-21
Sample_ID	BGHcLV52-45	BGHcLV52-46
RBP	RPL8	RPL8
Batch_ID	BGHcLV52	BGHcLV52
WB_result	63.700	50.800
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4866	4867

Sequencing Information

	BGHcLV52-45	BGHcLV52-46
Sample_ID	BGHcLV52-45	BGHcLV52-46
Sample Name	RPL8-BGHcLV52-45	RPL8-BGHcLV52-46
Pool ID	Pool-260528	Pool-260528
LocalServer_folder	set82	set82
total_reads	37,171,422	42,441,565
total_aligned_reads	31,619,912	39,970,226
unique_aligned_reads	30,099,880	37,970,928
percent_uniqueAligned	0.80976	0.89466
correlation_replicates	0.998490	0.998490
spikein_reads	55,460	54,208
percent_spikeins	0.00149	0.00128
original_ReadLength	151	151
QC_Status	ReadyToSubmit	ReadyToSubmit
ID	3567	3568

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RPL8-BGHcLV52-45_R1.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3567	NovaSeq6000	100	paired-ended	1				NT-BGHcLV52-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGHcLV52-2_R1.filtered.trimmed.paired.fastq.gz	ENCORE2	33690
RPL8-BGHcLV52-45_R2.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3567	NovaSeq6000	100	paired-ended	2				NT-BGHcLV52-1_R2.filtered.trimmed.paired.fastq.gz,NT-BGHcLV52-2_R2.filtered.trimmed.paired.fastq.gz	ENCORE2	33748
RPL8-BGHcLV52-45_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3567					GRCh38	V40	RPL8-BGHcLV52-45_R1.filtered.trimmed.paired.fastq.gz,RPL8-BGHcLV52-45_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	33808
RPL8-BGHcLV52-45_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3567					GRCh38	V40	RPL8-BGHcLV52-45_Aligned.sortedByCoord.out.bam		ENCORE2	33866
RPL8-BGHcLV52-45_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3567					GRCh38	V40	RPL8-BGHcLV52-45_Aligned.sortedByCoord.out.bam		ENCORE2	33924
RPL8-BGHcLV52-45_Signal.Unique.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3567					GRCh38	V40	RPL8-BGHcLV52-45_Aligned.sortedByCoord.out.bam		ENCORE2	33982
RPL8-BGHcLV52-45_Signal.Unique.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3567					GRCh38	V40	RPL8-BGHcLV52-45_Aligned.sortedByCoord.out.bam		ENCORE2	34040
RPL8-BGHcLV52-45_quant.sf	ENCODE_DATA/set82/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3567					GRCh38	V40	RPL8-BGHcLV52-45_R1.filtered.trimmed.paired.fastq.gz,RPL8-BGHcLV52-45_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	34098
RPL8-BGHcLV52-46_R1.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3568	NovaSeq6000	100	paired-ended	1				NT-BGHcLV52-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGHcLV52-2_R1.filtered.trimmed.paired.fastq.gz	ENCORE2	33691
RPL8-BGHcLV52-46_R2.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3568	NovaSeq6000	100	paired-ended	2				NT-BGHcLV52-1_R2.filtered.trimmed.paired.fastq.gz,NT-BGHcLV52-2_R2.filtered.trimmed.paired.fastq.gz	ENCORE2	33749
RPL8-BGHcLV52-46_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3568					GRCh38	V40	RPL8-BGHcLV52-46_R1.filtered.trimmed.paired.fastq.gz,RPL8-BGHcLV52-46_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	33809
RPL8-BGHcLV52-46_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3568					GRCh38	V40	RPL8-BGHcLV52-46_Aligned.sortedByCoord.out.bam		ENCORE2	33867
RPL8-BGHcLV52-46_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3568					GRCh38	V40	RPL8-BGHcLV52-46_Aligned.sortedByCoord.out.bam		ENCORE2	33925
RPL8-BGHcLV52-46_Signal.Unique.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3568					GRCh38	V40	RPL8-BGHcLV52-46_Aligned.sortedByCoord.out.bam		ENCORE2	33983
RPL8-BGHcLV52-46_Signal.Unique.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3568					GRCh38	V40	RPL8-BGHcLV52-46_Aligned.sortedByCoord.out.bam		ENCORE2	34041
RPL8-BGHcLV52-46_quant.sf	ENCODE_DATA/set82/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3568					GRCh38	V40	RPL8-BGHcLV52-46_R1.filtered.trimmed.paired.fastq.gz,RPL8-BGHcLV52-46_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	34099