non-target      CRISPR in HepG2      Batch: BGHcLV52

General Information

List KD experiments controlled by BGHcLV52-1 and BGHcLV52-2


NT-BGHcLV52-1,NT-BGHcLV52-2:No KD experiments controlled by NT-BGHcLV52-1 and NT-BGHcLV52-2 are available on the database




Experiment Information (Status: Sequenced)
BGHcLV52-1BGHcLV52-2
idx19391940
TRCN#_or_BGC#BGC#0000000BGC#0000000
shRNA_or_gRNA_sequenceCAGTCGGGCGTCATCATGATCAGTCGGGCGTCATCATGAT
PAM
Namenon-targetnon-target
Sample_IDBGHcLV52-1BGHcLV52-2
transduction_Date1/8/261/8/26
daysD6D6
RBP_namenon-targetnon-target
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc03095
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID1012810129




Western Blot
Western Blot info was not avaliable




Experiment Status
BGHcLV52-1BGHcLV52-2
Sample_IDBGHcLV52-1BGHcLV52-2
Sample Name
Sample NameNT-BGHcLV52-1NT-BGHcLV52-2
RBPnon-targetnon-target
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2026-09-162026-09-16
ProjectENCORE2ENCORE2
Note
ID1468814689




Library-Prep Information
BGHcLV52-1BGHcLV52-2
Sample #2122
Sample NameNT-BGHcLV52-1NT-BGHcLV52-2
Sample_Name_Alias
Index Well PositionE03F03
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp285281
Peak_Molarity60.3064.20
libSampleQC_DNA_WellDNA_Library_set82/set1/E10.pngDNA_Library_set82/set1/F10.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set82/set1/E3.pngRNA_Library_set82/set1/F3.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGHcLV52-1BGHcLV52-2
RBPNTNT
Batch_IDBGHcLV52BGHcLV52
WB_result0.0000.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48604861




Sequencing Information
BGHcLV52-1BGHcLV52-2
Sample_IDBGHcLV52-1BGHcLV52-2
Sample NameNT-BGHcLV52-1NT-BGHcLV52-2
Pool IDPool-260528Pool-260528
LocalServer_folderset82set82
total_reads44,246,43037,854,267
total_aligned_reads38,444,14832,142,558
unique_aligned_reads36,514,64530,594,955
percent_uniqueAligned0.825260.80823
correlation_replicates0.9988840.998884
spikein_reads61,17462,544
percent_spikeins0.001380.00165
original_ReadLength151151
QC_StatusReadyToSubmitReadyToSubmit
ID35613562




Data Submission Information
DCC submission information was not avaliable




File Information
file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
NT-BGHcLV52-1_R1.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3561NovaSeq6000100paired-ended1ENCORE233678
NT-BGHcLV52-1_R2.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3561NovaSeq6000100paired-ended2ENCORE233736
NT-BGHcLV52-1_Aligned.sortedByCoord.out.bamENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3561GRCh38V40NT-BGHcLV52-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGHcLV52-1_R2.filtered.trimmed.paired.fastq.gzENCORE233794
NT-BGHcLV52-1_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3561GRCh38V40NT-BGHcLV52-1_Aligned.sortedByCoord.out.bamENCORE233852
NT-BGHcLV52-1_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3561GRCh38V40NT-BGHcLV52-1_Aligned.sortedByCoord.out.bamENCORE233910
NT-BGHcLV52-1_Signal.Unique.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3561GRCh38V40NT-BGHcLV52-1_Aligned.sortedByCoord.out.bamENCORE233968
NT-BGHcLV52-1_Signal.Unique.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3561GRCh38V40NT-BGHcLV52-1_Aligned.sortedByCoord.out.bamENCORE234026
NT-BGHcLV52-1_quant.sfENCODE_DATA/set82/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3561GRCh38V40NT-BGHcLV52-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGHcLV52-1_R2.filtered.trimmed.paired.fastq.gzENCORE234084
NT-BGHcLV52-2_R1.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3562NovaSeq6000100paired-ended1ENCORE233679
NT-BGHcLV52-2_R2.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3562NovaSeq6000100paired-ended2ENCORE233737
NT-BGHcLV52-2_Aligned.sortedByCoord.out.bamENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3562GRCh38V40NT-BGHcLV52-2_R1.filtered.trimmed.paired.fastq.gz,NT-BGHcLV52-2_R2.filtered.trimmed.paired.fastq.gzENCORE233795
NT-BGHcLV52-2_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3562GRCh38V40NT-BGHcLV52-2_Aligned.sortedByCoord.out.bamENCORE233853
NT-BGHcLV52-2_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3562GRCh38V40NT-BGHcLV52-2_Aligned.sortedByCoord.out.bamENCORE233911
NT-BGHcLV52-2_Signal.Unique.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3562GRCh38V40NT-BGHcLV52-2_Aligned.sortedByCoord.out.bamENCORE233969
NT-BGHcLV52-2_Signal.Unique.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3562GRCh38V40NT-BGHcLV52-2_Aligned.sortedByCoord.out.bamENCORE234027
NT-BGHcLV52-2_quant.sfENCODE_DATA/set82/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3562GRCh38V40NT-BGHcLV52-2_R1.filtered.trimmed.paired.fastq.gz,NT-BGHcLV52-2_R2.filtered.trimmed.paired.fastq.gzENCORE234085