SMG8      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-99BGHcLV49-100
idx00
TRCN#_or_BGC#BGC#0000873BGC#0000873
shRNA_or_gRNA_sequenceCGCGGAAGGTAACCGAACTGCGCGGAAGGTAACCGAACTG
PAMAGGAGG
NameSMG8_97SMG8_97
Sample_IDBGHcLV49-99BGHcLV49-100
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameSMG8SMG8
qPCR_result21.911.6
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc51074302
WB_result59.377.1
Ave_WB
WB_DONE_date7/10/25
MW110kd
IP
antibody_Cat#A301-471ALOT #1
Antibody DCC IDENCAB236BQW
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID99839984




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SMG8Product_ID: A301-471A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SMG8-human		 SMG8-hepg2-CRISPR-A301-471A-LICOR.png<br>Caption: Western blot following CRISPR against SMG8 in HepG2 whole cell lysate using SMG8 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMG8. SMG8 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SMG8-K562-CRISPR-A301-471A.png<br>Caption: Western blot following CRISPR against SMG8 in K562 whole cell lysate using SMG8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SMG8. SMG8 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-99BGHcLV49-100
Sample_IDBGHcLV49-99BGHcLV49-100
Sample Name
Sample Name
RBPSMG8SMG8
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-122025-08-12
ProjectENCORE2ENCORE2
Note
ID1436414365




Library-Prep Information
BGHcLV49-99BGHcLV49-100
Sample #6162
Sample NameSMG8-BGHcLV49-99SMG8-BGHcLV49-100
Sample_Name_Alias
Index Well PositionG08H08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp264251
Peak_Molarity68.0052.50
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-09-102025-09-10
Sample_IDBGHcLV49-99BGHcLV49-100
RBPSMG8SMG8
Batch_IDBGHcLV49BGHcLV49
WB_result59.30077.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSampleQCedSampleQCed
ProjectENCORE2ENCORE2
ID48064807




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database