WDR77      CRISPR in HepG2      Batch: BGHcLV47

Experiment Information (Status: Sequenced)
BGHcLV47-89BGHcLV47-90
idx00
TRCN#_or_BGC#BGC#0001208BGC#0001208
shRNA_or_gRNA_sequenceCAACGTGGTGGACGACCTGACAACGTGGTGGACGACCTGA
PAMTGGTGG
NameWDR77_91WDR77_91
Sample_IDBGHcLV47-89BGHcLV47-90
transduction_Date1/28/251/28/25
daysD6D6
RBP_nameWDR77WDR77
qPCR_result7.427.1
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc40934473
WB_result62.465.6
Ave_WB
WB_DONE_date2/6/25
MW42kd
IP
antibody_Cat#2018Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98769877




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
WDR77Product_ID: 2018S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: WDR77-human		 WDR77-HEPG2-CRISPR-2018S.png<br>Caption: Western blot following CRISPR against WDR77 in HepG2 whole cell lysate using WDR77 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against WDR77. WDR77 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
 CST_2018S_1_WDR77.png<br>Caption: IP-WB analysis of 2018S whole cell lysate using the WDR77 specific antibody, 2018S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the WDR77-specific antibody, 2018S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 WDR77-K562-CRISPR-2018S.png<br>Caption: Western blot following CRISPR against WDR77 in K562 whole cell lysate using WDR77 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against WDR77. WDR77 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGHcLV47-89BGHcLV47-90
Sample_IDBGHcLV47-89BGHcLV47-90
Sample Name
Sample NameWDR77-BGHcLV47-89WDR77-BGHcLV47-90
RBPWDR77WDR77
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-05-142025-05-14
ProjectENCORE2ENCORE2
Note
ID1418014181




Library-Prep Information
BGHcLV47-89BGHcLV47-90
Sample #3334
Sample NameWDR77-BGHcLV47-89WDR77-BGHcLV47-90
Sample_Name_Alias
Index Well PositionA12B12
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-04-232025-04-23
Lib_IDLib-250423Lib-250423
Tecan_Location
Tecan
Tecan_date
Size_bp257260
Peak_Molarity66.9083.10
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-04-222025-04-22
Sample_IDBGHcLV47-89BGHcLV47-90
RBPWDR77WDR77
Batch_IDBGHcLV47BGHcLV47
WB_result62.40065.600
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47444745




Sequencing Information
BGHcLV47-89BGHcLV47-90
Sample_IDBGHcLV47-89BGHcLV47-90
Sample NameWDR77-BGHcLV47-89WDR77-BGHcLV47-90
Pool IDPool-250422Pool-250422
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34473448




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database