RPS3      CRISPR in HepG2      Batch: BGHcLV47

Experiment Information (Status: Sequenced)
BGHcLV47-61BGHcLV47-62
idx00
TRCN#_or_BGC#BGC#0000048BGC#0000048
shRNA_or_gRNA_sequenceCTTTCCAGAGGGCAGTGTAGCTTTCCAGAGGGCAGTGTAG
PAM
NameRPS3-3RPS3-3
Sample_IDBGHcLV47-61BGHcLV47-62
transduction_Date1/28/251/28/25
daysD6D6
RBP_nameRPS3RPS3
qPCR_result66.762.6
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc32043213
WB_result50.161.9
Ave_WB
WB_DONE_date3/25/25
MW27 kDa
IP1
antibody_Cat#A303-840A
Antibody DCC IDENCAB855QRN
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98489849




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPS3Product_ID: A303-840A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPS3-human		 HepG2_Bethyl_A303-840A_1_RPS3.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using RPS3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RPS3 antibody (lanes under 'RPS3').
 RPS3-HEPG2-CRISPR-A303-840A.png<br>Caption: Western blot following CRISPR against RPS3 in HepG2 whole cell lysate using RPS3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS3. RPS3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 K562_Bethyl_A303-840A_1_RPS3.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using RPS3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RPS3 antibody (lanes under 'RPS3').
 RPS3-K562-crispr.png<br>Caption: Western blot following CRISPR against RPS3 in K562 whole cell lysate using RPS3 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS3. RPS3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.




Experiment Status
BGHcLV47-61BGHcLV47-62
Sample_IDBGHcLV47-61BGHcLV47-62
Sample Name
Sample NameRPS3-BGHcLV47-61RPS3-BGHcLV47-62
RBPRPS3RPS3
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-05-142025-05-14
ProjectENCORE2ENCORE2
Note
ID1417414175




Library-Prep Information
BGHcLV47-61BGHcLV47-62
Sample #2728
Sample NameRPS3-BGHcLV47-61RPS3-BGHcLV47-62
Sample_Name_Alias
Index Well PositionC11D11
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-04-232025-04-23
Lib_IDLib-250423Lib-250423
Tecan_Location
Tecan
Tecan_date
Size_bp274280
Peak_Molarity91.2086.60
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-04-222025-04-22
Sample_IDBGHcLV47-61BGHcLV47-62
RBPRPS3RPS3
Batch_IDBGHcLV47BGHcLV47
WB_result50.10061.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47384739




Sequencing Information
BGHcLV47-61BGHcLV47-62
Sample_IDBGHcLV47-61BGHcLV47-62
Sample NameRPS3-BGHcLV47-61RPS3-BGHcLV47-62
Pool IDPool-250422Pool-250422
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34413442




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database