GFPT1      CRISPR in HepG2      Batch: BGHcLV45

Experiment Information (Status: Sequencing)
BGHcLV45-39BGHcLV45-40
idx12171217
TRCN#_or_BGC#BGC#0001142BGC#0001142
shRNA_or_gRNA_sequenceTGGAATAGCTCATACCCGTTTGGAATAGCTCATACCCGTT
PAMGGGGGG
NameGFPT1_95GFPT1_95
Sample_IDBGHcLV45-39BGHcLV45-40
transduction_Date10/1/2410/1/24
daysD6D6
RBP_nameGFPT1GFPT1
qPCR_result92.189.4
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc46534700
WB_result74.278.0
Ave_WB
WB_DONE_date10/10/24
MW80kd
IP
antibody_Cat#5322Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID76817682




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
GFPT1Product_ID: 5322S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: GFPT1-human		 GFPT1-HEPG2-CRISPR-5322S.png<br>Caption: Western blot following CRISPR against GFPT1 in HepG2 whole cell lysate using GFPT1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GFPT1. GFPT1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_5322S_1_GFPT1.png<br>Caption: IP-WB analysis of 5322S whole cell lysate using the GFPT1 specific antibody, 5322S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the GFPT1-specific antibody, 5322S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 GFPT1-K562-CRISPR-5322S.png<br>Caption: Western blot following CRISPR against GFPT1 in K562 whole cell lysate using GFPT1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GFPT1. GFPT1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV45-39BGHcLV45-40
Sample_IDBGHcLV45-39BGHcLV45-40
Sample Name
Sample NameGFPT1-BGHcLV45-39GFPT1-BGHcLV45-40
RBPGFPT1GFPT1
Cell_LineHepG2HepG2
Exp UID
StatusSequencingSequencing
Status_date2024-12-122024-12-12
ProjectENCORE2ENCORE2
Note
ID1370613707




Library-Prep Information
BGHcLV45-39BGHcLV45-40
Sample #2122
Sample NameGFPT1-BGHcLV45-39GFPT1-BGHcLV45-40
Sample_Name_Alias
Index Well PositionE09F09
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-12-022024-12-02
Lib_IDLib-241202Lib-241202
Tecan_Location
Tecan
Tecan_date
Size_bp288283
Peak_Molarity78.6081.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-12-052024-12-05
Sample_IDBGHcLV45-39BGHcLV45-40
RBPGFPT1GFPT1
Batch_IDBGHcLV45BGHcLV45
WB_result74.20078.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46304631




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database