AGO2      CRISPR in HepG2      Batch: BGHcLV07

Experiment Information (Status: NotSatisfied)
BGHcLV07-3BGHcLV07-4
idx115116
TRCN#_or_BGC#BGC#0000077BGC#0000077
shRNA_or_gRNA_sequenceGAGGTCCCAAAGTCGGGTCTGAGGTCCCAAAGTCGGGTCT
PAM
NameAGO2(EIF2C2)_86_3AGO2(EIF2C2)_86_3
Sample_IDBGHcLV07-3BGHcLV07-4
transduction_Date6/13/176/13/17
daysD6D6
RBP_nameAGO2AGO2
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc35293672
WB_result0.04.3
Ave_WB2.2
WB_DONE_date7/5/17
MW97kD
IPFU'S
antibody_Cat#H00027161-M01
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM100
Rep2_TPM70
Action
Library_start_date
repeat_library
Note
ID62566257




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
AGO2Product_ID: H00027161-M01
Lot_ID: E8251-S2
Source: Abnova
Target Name: AGO2-human		 AGO2_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AGO2/EIF2C2 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-AGO2/EIF2C2 antibody (lanes under 'anti-AGO2'). Asterisk indicates heavy and light chains of antibody.
 AGO2-HEPG2_FU's.png<br>Caption: Western blot following CRISPR against AGO2 in HepG2 whole cell lysate using AGO2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against AGO2. AGO2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 AGO2_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using AGO2/EIF2C2 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-AGO2/EIF2C2 antibody (lanes under 'anti-AGO2'). Asterisk indicates heavy and light chains of antibody.




Experiment Status
BGHcLV07-3BGHcLV07-4
Sample_IDBGHcLV07-3BGHcLV07-4
Sample Name
Sample Name
RBPAGO2AGO2
Cell_LineHepG2HepG2
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2019-07-082019-07-08
ProjectENCODE3ENCODE3
Note
ID25232524




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database