AGO2 antibody: H00027161-M01


Antibody Info
Product_IDH00027161-M01
Lot_IDE8251-S2
Host_organismmouse
SourceAbnova
Antibody_DCC_IDENCAB126OWL
Target Info
GeneNameAGO2
Phosphate
Target_nameAGO2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 AGO2_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AGO2/EIF2C2 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-AGO2/EIF2C2 antibody (lanes under 'anti-AGO2'). Asterisk indicates heavy and light chains of antibody.

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AGO2/EIF2C2 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-AGO2/EIF2C2 antibody (lanes under 'anti-AGO2'). Asterisk indicates heavy and light chains of antibody.

Method: immunoprecipitation

Status: Released

Lab: Fu Lab

AGO2-HEPG2_FU's.png<br>Caption: Western blot following CRISPR against AGO2 in HepG2 whole cell lysate using AGO2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against AGO2. AGO2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against AGO2 in HepG2 whole cell lysate using AGO2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against AGO2. AGO2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHcLV07-3

Status: Released

Lab: Graveley Lab

K562 AGO2_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using AGO2/EIF2C2 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-AGO2/EIF2C2 antibody (lanes under 'anti-AGO2'). Asterisk indicates heavy and light chains of antibody.

Caption: IP-Western Blot analysis of K562 whole cell lysate using AGO2/EIF2C2 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-AGO2/EIF2C2 antibody (lanes under 'anti-AGO2'). Asterisk indicates heavy and light chains of antibody.

Method: immunoprecipitation

Releated Sample: eCLIP:4116

Status: Released

Lab: Fu Lab