GSPT2

General Information

Gene Name       : GSPT2
Gene ID             : ENSG00000189369.8
Essential Gene : No

Functions

Localization

Domains (AlphaFold: GSPT2)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878GSPT2ENSG00000189369.88.157.397.77ENCSR000AEF
HepG2GSPT2ENSG00000189369.80.000.000.00ENCSR985KAT
K562GSPT2ENSG00000189369.82.432.722.58ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq GSPT2-BGKcLV17-K562ENCSR148XGXGSPT2K562ReleasedENCSR516TLX   CRISPRKD% = 65.8,55.9951325






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
GSPT2ENCAB053JZLRN119PW1NotSatisfied, ENCODE4BGKcLV17-27, NotSatisfied, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
GSPT2K562CRISPRENCSR148XGXENCSR516TLXGSPT2-BGKcLV17-27,GSPT2-BGKcLV17-28NT-BGKcLV17-1,NT-BGKcLV17-265.8,55.9951325ReleasedENCODE4
GSPT2HepG2CRISPRBGHcLV25-37,BGHcLV25-38BGHcLV25-1,BGHcLV25-2No clear band on the position,NotSatisfiedENCODE4
GSPT2HepG2CRISPRBGHcLV25-39,BGHcLV25-40BGHcLV25-1,BGHcLV25-2No clear band on the position,NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
GSPT2Product_ID: RN119PW
Lot_ID: 1
Source: MBLI
Target Name: GSPT2-human		 MBL_RN119PW_001_GSPT2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the GSPT2 specific antibody, RN119PW. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the GSPT2-specific antibody, RN119PW. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 GSPT2-K562-CRISPR-RN119PW.png<br>Caption: Western blot following CRISPR against GSPT2 in K562 whole cell lysate using GSPT2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSPT2. GSPT2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for GSPT2 is not avaliable on ENCORE database.



Proteins bound to RNA

  • NO known proteins bind on GSPT2 RNA with finished eCLIP experiments on ENCORE database.





eCLIP assay for GSPT2 is not avaliable on ENCORE database.






NO known proteins are bound to GSPT2 RNA with finished eCLIP experiments on ENCORE database.





eCLIP assay for GSPT2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for GSPT2 is not available on ENCORE database.

  • 11 RBPs can affect the levels of GSPT2.  








The Knockdown/knockout RNA-Seq assay for GSPT2 is not available on ENCORE database.





11 RBPs can affect the gene expression of GSPT2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
GSPT2ENSG00000189369.751.47310118.11550-1.198304.837804.12180K562AGGF1
GSPT2ENSG00000189369.766.12070122.90290-0.894302.555101.92640K562AKAP8L
GSPT2ENSG00000189369.7382.42650164.148501.2201010.447709.41730K562AQR
GSPT2ENSG00000189369.7196.44770296.36960-0.593303.045102.02350K562CELF1
GSPT2ENSG00000189369.7118.5378058.246201.025002.652502.05800K562FXR1
GSPT2ENSG00000189369.7108.3090049.130801.140403.952803.07060K562G3BP2
GSPT2ENSG00000189369.781.21320130.43640-0.683602.069501.36650K562KIF1C
GSPT2ENSG00000189369.7188.1052091.294601.042804.915603.91880K562PRPF8
GSPT2ENSG00000189369.751.68970121.89760-1.237704.784404.09530K562PUS1
GSPT2ENSG00000189369.763.00170118.76120-0.914603.291202.65330K562RBM15
GSPT2ENSG00000189369.7103.2101049.176201.069504.021103.05340K562U2AF2
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for GSPT2 is not available on ENCORE database.






No RBPs affect the alternative splcing events of GSPT2 on ENCORE database.





The Knockdown/knockout RNA-Seq assay for GSPT2 is not avaliable on ENCORE database.