| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPU specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either mouse monoclonal hnRNP U antibody (lanes under 'HNRNPU') or using mouse normal IgG (lanes under 'IgG'). Method: immunoprecipitation Releated Sample: eCLIP:281 Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against HNRNPU in K562 and HepG2 whole cell lysate using HNRNPU specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPU. Lanes 5-8 follow the same pattern, but in HepG2. HNRNPU protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV21-23 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-WB characterization of HNRNPU specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using mouse monoclonal hnRNP U Antibody (3G6). This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Method: immunoprecipitation Releated Sample: eCLIP:249 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against HNRNPU in K562 and HepG2 whole cell lysate using HNRNPU specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPU. Lanes 5-8 follow the same pattern, but in HepG2. HNRNPU protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV21-23 Status: Released Lab: Graveley Lab |
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