SF3B1 antibody: ab66774


Antibody Info
Product_IDab66774
Lot_IDGR216813-1
Host_organismrabbit
SourceAbcam
Antibody_DCC_IDENCAB022ALQ
Target Info
GeneNameSF3B1
Phosphate
Target_nameSF3B1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 SF3B1-HEPG2.png<br>Caption: Western blot following shRNA against SF3B1 in HepG2 whole cell lysate using SF3B1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B1.SF3B1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against SF3B1 in HepG2 whole cell lysate using SF3B1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B1.SF3B1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV35-41

Status: Released

Lab: Graveley Lab

K562 K562_Abcam_ab66774_GR216813-1_SF3B1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using SF3B1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B1 antibody (lanes under 'SF3B1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using SF3B1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B1 antibody (lanes under 'SF3B1').

Method: immunoprecipitation

Releated Sample: eCLIP:506

Status: Released

Lab: Yeo Lab

SF3B1-K562-CRISPR.png<br>Caption: Western blot following CRISPR against SF3B1 in K562 whole cell lysate using SF3B1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SF3B1.SF3B1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against SF3B1 in K562 whole cell lysate using SF3B1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SF3B1.SF3B1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKcLV11-93

Status: Released

Lab: Graveley Lab