| HepG2 | 
Caption: Representative image of immunoprecipitation performed on whole cell extracts from the HepG2 cell line using the MATR3 specific antibody ab151714. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using MATR3 antibody. Lane 4: Immunoprecipitated material using MATR3 antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 94.62kDa. Method: immunoprecipitation Releated Sample: eCLIP:695 Status: Released Lab: Yeo Lab 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using MATR3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lanes 4 is 10% IP enrichment using rabbit polyclonal MATR3 antibody (lanes under 'MATR3'). Method: immunoprecipitation Releated Sample: eCLIP:695 Status: Released Lab: Yeo Lab | 
Caption: IP followed by mass spectrometry. Protein was immunoprecipitated from HepG2 whole cell lysates using the antibody ab151714, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility. Status: Released Lab: Yeo Lab |
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| K562 | 
Caption: Representative image of immunoprecipitation performed on whole cell extracts from the K562 cell line using the MATR3-specific antibody ab151714. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using MATR3 antibody. Lane 4: Immunoprecipitated material using MATR3 antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 94.62 kDa. Method: immunoprecipitation Releated Sample: eCLIP:716 Status: Released Lab: Yeo Lab 
Caption: IP-Western Blot analysis of K562 whole cell lysate using MATR3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lanes 4 and 5 are 10% IP enrichment of biological replicates using rabbit polyclonal MATR3 antibody (lanes under 'MATR3'). Method: immunoprecipitation Releated Sample: eCLIP:716 Status: Released Lab: Yeo Lab |
Caption: IP followed by mass spectrometry. Protein was immunoprecipitated from K562 whole cell lysates using the antibody ab151714, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility. Status: NotReviewed Lab: Yeo Lab |
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