FTO antibody: RN121PW


Antibody Info
Product_IDRN121PW
Lot_ID1
Host_organismrabbit
SourceMBLI
Antibody_DCC_IDENCAB377UYD
Target Info
GeneNameFTO
Phosphate
Target_nameFTO-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_MBLI_RN121PW_001_FTO.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FTO specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FTO antibody (lanes under 'FTO').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FTO specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FTO antibody (lanes under 'FTO').

Method: immunoprecipitation

Releated Sample: eCLIP:723

Status: Released

Lab: Yeo Lab

FTO-HEPG2.png<br>Caption: Western blot following shRNA against FTO in HepG2 whole cell lysate using FTO specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FTO.FTO protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against FTO in HepG2 whole cell lysate using FTO specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FTO.FTO protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV35-17

Status: Released

Lab: Graveley Lab

K562 K562_MBLI_RN121PW_001_FTO.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using FTO specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FTO antibody (lanes under 'FTO').

Caption: IP-Western Blot analysis of K562 whole cell lysate using FTO specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FTO antibody (lanes under 'FTO').

Method: immunoprecipitation

Releated Sample: eCLIP:464

Status: Released

Lab: Yeo Lab

FTO-K562.png<br>Caption: Western blot following shRNA against FTO in k562 whole cell lysate using FTO specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FTO.FTO protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against FTO in k562 whole cell lysate using FTO specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FTO.FTO protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKLV36-25

Status: Released

Lab: Graveley Lab