SRSF7 antibody: RN079PW


Antibody Info
Product_IDRN079PW
Lot_ID1
Host_organismrabbit
SourceMBLI
Antibody_DCC_IDENCAB910DVS
Target Info
GeneNameSRSF7
Phosphate
Target_nameSRSF7-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_MBLI_RN079PW_001_SRSF7.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SRSF7 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SRSF7 antibody (lanes under 'SRSF7').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SRSF7 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SRSF7 antibody (lanes under 'SRSF7').

Method: immunoprecipitation

Releated Sample: eCLIP:209

Status: Released

Lab: Yeo Lab

SRSF7_Secondary_Western.png<br>Caption: Western blot following shRNA against SRSF7 in K562 and HepG2 whole cell lysate using SRSF7 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against SRSF7. Lanes 5-7 follow the same pattern, but in HepG2. SRSF7 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against SRSF7 in K562 and HepG2 whole cell lysate using SRSF7 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against SRSF7. Lanes 5-7 follow the same pattern, but in HepG2. SRSF7 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV11-53

Status: Released

Lab: Graveley Lab

SRSF7-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against SRSF7 in HepG2 whole cell lysate using SRSF7 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF7. SRSF7 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following CRISPR against SRSF7 in HepG2 whole cell lysate using SRSF7 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF7. SRSF7 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHcLV03-5

Status: Released

Lab: Graveley Lab

K562 K562_MBLI_RN079PW_001_SRSF7.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using SRSF7 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SRSF7 antibody (lanes under 'SRSF7').

Caption: IP-Western Blot analysis of K562 whole cell lysate using SRSF7 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SRSF7 antibody (lanes under 'SRSF7').

Method: immunoprecipitation

Releated Sample: eCLIP:376

Status: Released

Lab: Yeo Lab

SRSF7_Secondary_Western.png<br>Caption: Western blot following shRNA against SRSF7 in K562 and HepG2 whole cell lysate using SRSF7 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against SRSF7. Lanes 5-7 follow the same pattern, but in HepG2. SRSF7 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against SRSF7 in K562 and HepG2 whole cell lysate using SRSF7 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against SRSF7. Lanes 5-7 follow the same pattern, but in HepG2. SRSF7 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV11-53

Status: Released

Lab: Graveley Lab

SRSF7-K562-CRISPR.png<br>Caption: Western blot following CRISPR against SRSF7 in k562 whole cell lysate using SRSF7 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF7. SRSF7 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following CRISPR against SRSF7 in k562 whole cell lysate using SRSF7 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF7. SRSF7 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKcLV02-5

Status: Released

Lab: Graveley Lab