TIAL1 antibody: RN059PW


Antibody Info
Product_IDRN059PW
Lot_ID1
Host_organismrabbit
SourceMBLI
Antibody_DCC_IDENCAB050ZSG
Target Info
GeneNameTIAL1
Phosphate
Target_nameTIAL1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_MBL_RN059PW_001_TIAL1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using TIAL1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TIAL1 antibody (lanes under 'TIAL1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using TIAL1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TIAL1 antibody (lanes under 'TIAL1').

Method: immunoprecipitation

Releated Sample: eCLIP:753

Status: Released

Lab: Yeo Lab

TIAL1_Secondary_Western.png<br>Caption: Western blot following shRNA against TIAL1 in K562 and HepG2 whole cell lysate using TIAL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TIAL1. Lanes 5-8 follow the same pattern, but in HepG2. TIAL1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against TIAL1 in K562 and HepG2 whole cell lysate using TIAL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TIAL1. Lanes 5-8 follow the same pattern, but in HepG2. TIAL1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV21-61

Status: Released

Lab: Graveley Lab

K562 TIAL1_Secondary_Western.png<br>Caption: Western blot following shRNA against TIAL1 in K562 and HepG2 whole cell lysate using TIAL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TIAL1. Lanes 5-8 follow the same pattern, but in HepG2. TIAL1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against TIAL1 in K562 and HepG2 whole cell lysate using TIAL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TIAL1. Lanes 5-8 follow the same pattern, but in HepG2. TIAL1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV21-61

Status: Released

Lab: Graveley Lab