Antibodies of TIAL1


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
TIAL1Product_ID: RN059PW
Lot_ID: 1
Source: MBLI
Target Name: TIAL1-human
HepG2_MBL_RN059PW_001_TIAL1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using TIAL1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TIAL1 antibody (lanes under 'TIAL1').
TIAL1_Secondary_Western.png<br>Caption: Western blot following shRNA against TIAL1 in K562 and HepG2 whole cell lysate using TIAL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TIAL1. Lanes 5-8 follow the same pattern, but in HepG2. TIAL1 protein appears as the green band, Tubulin serves as a control and appears in red.
TIAL1_Secondary_Western.png<br>Caption: Western blot following shRNA against TIAL1 in K562 and HepG2 whole cell lysate using TIAL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TIAL1. Lanes 5-8 follow the same pattern, but in HepG2. TIAL1 protein appears as the green band, Tubulin serves as a control and appears in red.
TIAL1Product_ID: 8509S
Lot_ID: 2
Source: Cell Signaling Technology
Target Name: TIAL1-human
CST_8509S_2_TIAL1.png<br>Caption: IP-WB analysis of 8509S whole cell lysate using the TIAL1 specific antibody, 8509S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the TIAL1-specific antibody, 8509S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
TIAL1-K562-8509S.png<br>Caption: Western blot following shRNA against TIAL1 in K562 whole cell lysate using TIAL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against TIAL1. TIAL1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
TIAL1Product_ID: 8611S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: TIAL1-human
CST_8611S_1_TIAL1.png<br>Caption: IP-WB analysis of 8611S whole cell lysate using the TIAL1 specific antibody, 8611S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the TIAL1-specific antibody, 8611S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
TIAL1-K562-8611S.png<br>Caption: Western blot following shRNA against TIAL1 in K562 whole cell lysate using TIAL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against TIAL1. TIAL1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.