| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPC specific antibody. Lane 1 is 1% of twenty million whole cell lysate input, lane 2 is 1% supernatant after immunoprecipitation and lane 3 is 10% of IP enrichment using rabbit polyclonal anti-HNRNPC antibody (lanes under 'HNRNPC'). Lane 4 is 1% of twenty million whole cell lysate input and lane 5 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Method: immunoprecipitation Releated Sample: eCLIP:203 Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against HNRNPC in K562 and HepG2 whole cell lysate using HNRNPC specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against HNRNPC. Lanes 5-7 follow the same pattern, but in HepG2. HNRNPC protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV21-19 Status: Released Lab: Graveley Lab |
|---|
| K562 | 
Caption: IP-WB characterization of HNRNPC specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-HNRNPCpAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Method: immunoprecipitation Releated Sample: eCLIP:699 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against HNRNPC in K562 and HepG2 whole cell lysate using HNRNPC specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against HNRNPC. Lanes 5-7 follow the same pattern, but in HepG2. HNRNPC protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV21-19 Status: Released Lab: Graveley Lab |
|---|