G3BP1 antibody: RN048PW


Antibody Info
Product_IDRN048PW
Lot_ID1
Host_organismrabbit
SourceMBLI
Antibody_DCC_IDENCAB411RDE
Target Info
GeneNameG3BP1
Phosphate
Target_nameG3BP1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_MBLI_RN048PW_001_G3BP1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using G3BP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-G3BP1 antibody (lanes under 'G3BP1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using G3BP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-G3BP1 antibody (lanes under 'G3BP1').

Method: immunoprecipitation

Releated Sample: eCLIP:712

Status: Released

Lab: Yeo Lab

G3BP1-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against G3BP1 in HepG2 whole cell lysate using G3BP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against G3BP1. G3BP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against G3BP1 in HepG2 whole cell lysate using G3BP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against G3BP1. G3BP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV12-33

Status: Released

Lab: Graveley Lab

K562 G3BP1-K562-CRISPR.png<br>Caption: Western blot following CRISPR against G3BP1 in K562 whole cell lysate using G3BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against G3BP1. G3BP1 protein appears as the green band, Beta-actin serves as a control and appears in red.

Caption: Western blot following CRISPR against G3BP1 in K562 whole cell lysate using G3BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against G3BP1. G3BP1 protein appears as the green band, Beta-actin serves as a control and appears in red.

Releated Sample: BGKcLV11-57

Status: Released

Lab: Graveley Lab