IGF2BP3 antibody: RN009P


Antibody Info
Product_IDRN009P
Lot_ID2
Host_organismrabbit
SourceMBLI
Antibody_DCC_IDENCAB934MDN
Target Info
GeneNameIGF2BP3
Phosphate
Target_nameIGF2BP3-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_MBLI_RN009P_002_IGF2BP3.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-IGF2BP3 antibody (lanes under 'IGF2BP3').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-IGF2BP3 antibody (lanes under 'IGF2BP3').

Method: immunoprecipitation

Releated Sample: eCLIP:211

Status: Released

Lab: Yeo Lab

IGF2BP3_Secondary_Western.png<br>Caption: Western blot following shRNA against IGF2BP3 in K562 and HepG2 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP3. Lanes follow the same pattern, but in HepG2. IGF2BP3 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against IGF2BP3 in K562 and HepG2 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP3. Lanes follow the same pattern, but in HepG2. IGF2BP3 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV11-19

Status: Released

Lab: Graveley Lab

K562 K562_MBLI_RN009P_002_IGF2BP3.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lanes 4 and 5 are 10% IP enrichments of biological replicates using rabbit polyclonal anti-IGF2BP3 antibody (lanes under 'IGF2BP3').

Caption: IP-Western Blot analysis of K562 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lanes 4 and 5 are 10% IP enrichments of biological replicates using rabbit polyclonal anti-IGF2BP3 antibody (lanes under 'IGF2BP3').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

IGF2BP3_Secondary_Western.png<br>Caption: Western blot following shRNA against IGF2BP3 in K562 and HepG2 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP3. Lanes follow the same pattern, but in HepG2. IGF2BP3 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against IGF2BP3 in K562 and HepG2 whole cell lysate using IGF2BP3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP3. Lanes follow the same pattern, but in HepG2. IGF2BP3 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV11-19

Status: Released

Lab: Graveley Lab