| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using IGF2BP2 specific antibody. Lane 1 is 2.5% of five million whole cell lysate input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either rabbit polyclonal anti-IGF2BP2 antibody (lanes under 'IGF2BP2') or using rabbit normal IgG (lanes under 'IgG'). Stars indicate light and heavy chains of IgG background Method: immunoprecipitation Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against IGF2BP2 in K562 and HepG2 whole cell lysate using IGF2BP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP2. Lanes 5-7 follow the same pattern, but in HepG2. IGF2BP2 protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV11-21 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using IGF2BP2 specific antibody. Lane 1 is 2.5% of five million whole cell lysate input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either rabbit polyclonal anti-IGF2BP2 antibody (lanes under 'IGF2BP2') or using rabbit normal IgG (lanes under 'IgG'). Stars indicate light and heavy chains of IgG background Method: immunoprecipitation Releated Sample: eCLIP:226 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against IGF2BP2 in K562 and HepG2 whole cell lysate using IGF2BP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP2. Lanes 5-7 follow the same pattern, but in HepG2. IGF2BP2 protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV11-21 Status: Released Lab: Graveley Lab |
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