EIF4G2 antibody: RN003P


Antibody Info
Product_IDRN003P
Lot_ID1
Host_organismrabbit
SourceMBLI
Antibody_DCC_IDENCAB000BFJ
Target Info
GeneNameEIF4G2
Phosphate
Target_nameEIF4G2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 EIF4G2_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF4G2 in K562 and HepG2 whole cell lysate using EIF4G2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF4G2. Lanes 5-8 follow the same pattern, but in HepG2. EIF4G2 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against EIF4G2 in K562 and HepG2 whole cell lysate using EIF4G2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF4G2. Lanes 5-8 follow the same pattern, but in HepG2. EIF4G2 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV25-17

Status: Released

Lab: Graveley Lab

K562 K562_MBLI_RN003P_001_EIF4G2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF4G2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4G2 antibody (lanes under 'EIF4G2').

Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF4G2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4G2 antibody (lanes under 'EIF4G2').

Method: immunoprecipitation

Releated Sample: eCLIP:260

Status: Released

Lab: Yeo Lab

EIF4G2_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF4G2 in K562 and HepG2 whole cell lysate using EIF4G2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF4G2. Lanes 5-8 follow the same pattern, but in HepG2. EIF4G2 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against EIF4G2 in K562 and HepG2 whole cell lysate using EIF4G2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF4G2. Lanes 5-8 follow the same pattern, but in HepG2. EIF4G2 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV25-17

Status: Released

Lab: Graveley Lab