Antibodies of EIF4G2


4 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF4G2Product_ID: PA5-21377
Lot_ID: TK2671326
Source: Thermo Fisher
Target Name: EIF4G2-human		 ThermoFisher_PA5-21377_TK2671326_DAP5.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DAP5 specific antibody, PA5-21377. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the DAP5-specific antibody, PA5-21377. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF4G2-K562-PA5-21377.png<br>Caption: Western blot following shRNA against EIF4G2 in K562 whole cell lysate using EIF4G2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against EIF4G2. EIF4G2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
EIF4G2Product_ID: RN003P
Lot_ID: 1
Source: MBLI
Target Name: EIF4G2-human		 EIF4G2_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF4G2 in K562 and HepG2 whole cell lysate using EIF4G2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF4G2. Lanes 5-8 follow the same pattern, but in HepG2. EIF4G2 protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_MBLI_RN003P_001_EIF4G2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF4G2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4G2 antibody (lanes under 'EIF4G2').
 EIF4G2_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF4G2 in K562 and HepG2 whole cell lysate using EIF4G2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF4G2. Lanes 5-8 follow the same pattern, but in HepG2. EIF4G2 protein appears as the green band, Tubulin serves as a control and appears in red.
EIF4G2Product_ID: 5169S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: EIF4G2-human		 CST_5169S_1_EIF4G2.png<br>Caption: IP-WB analysis of 5169S whole cell lysate using the EIF4G2 specific antibody, 5169S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF4G2-specific antibody, 5169S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
EIF4G2Product_ID: 702479
Lot_ID: NotAvailable
Source: Thermo Fisher
Target Name: EIF4G2-human		 EIF4G2-K562-702479.png<br>Caption: Western blot following shRNA against EIF4G2 in K562 whole cell lysate using EIF4G2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against EIF4G2. EIF4G2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.