FMR1 antibody: PA5-34584


Antibody Info
Product_IDPA5-34584
Lot_IDTK2671134
Host_organismrabbit
SourceThermo Fisher
Antibody_DCC_IDENCAB650WPE
Target Info
GeneNameFMR1
Phosphate
Target_nameFMR1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 FMR1-HEPG2-PA5-34584.png<br>Caption: Western blot following shRNA against FMR1 in HepG2 whole cell lysate using FMR1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against FMR1. FMR1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following shRNA against FMR1 in HepG2 whole cell lysate using FMR1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against FMR1. FMR1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHLV23-17

Status: Released

Lab: Graveley Lab

K562 ThermoFisher_PA5-34584_TK2671134_FMRP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the FMR1 specific antibody, PA5-34584. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the FMR1-specific antibody, PA5-34584. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the FMR1 specific antibody, PA5-34584. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the FMR1-specific antibody, PA5-34584. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Releated Sample: eCLIP:236, eCLIP:4030

Status: Released

Lab: Yeo Lab