| HepG2 | | 
Caption: Western blot following CRISPR against CD3EAP in HepG2 whole cell lysate using CD3EAP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CD3EAP. CD3EAP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow. Releated Sample: BGHcLV16-11 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-WB analysis of K562 whole cell lysate using the CD3EAP specific antibody, PA5-27632. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the CD3EAP-specific antibody, PA5-27632. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Comments: Immunoprecipitation lane (3) exhibits the same double banding pattern seen in another western blot example from the antibody supplier, though they are shifted much larger in size. We believe this is due to performing the characterization using the Jess Western Blotting system, which has a documented tendency for some proteins to run differently than with more traditional methods due to their compositions. Method: immunoprecipitation Status: NotSatisfied Lab: Yeo Lab | 
Caption: Western blot following CRISPR against CD3EAP in K562 whole cell lysate using CD3EAP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CD3EAP. CD3EAP protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKcLV15-9 Status: Released Lab: Graveley Lab |
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