Antibodies of CD3EAP


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CD3EAPProduct_ID: PA5-27632
Lot_ID: TL2692990
Source: Thermo Fisher
Target Name: CD3EAP-human		 CD3EAP-HEPG2-CRISPR-PA5-7632.png<br>Caption: Western blot following CRISPR against CD3EAP in HepG2 whole cell lysate using CD3EAP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CD3EAP. CD3EAP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_PA5-27632_TL2692990_CD3EAP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CD3EAP specific antibody, PA5-27632. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the CD3EAP-specific antibody, PA5-27632. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CD3EAP-K562-CRISPRE-PA5-27632.png<br>Caption: Western blot following CRISPR against CD3EAP in K562 whole cell lysate using CD3EAP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CD3EAP. CD3EAP protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
CD3EAPProduct_ID: A301-294A
Lot_ID: 2
Source: Bethyl Labs
Target Name: CD3EAP-human		 Bethyl_A301-294A_2_CD3EAP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CD3EAP specific antibody, A301-294A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CD3EAP-specific antibody, A301-294A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CD3EAP-K562-CRISPR-A301-294A.png<br>Caption: Western blot following CRISPR against CD3EAP in K562 whole cell lysate using CD3EAP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CD3EAP. CD3EAP protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.