PRPF39 antibody: PA5-21627


Antibody Info
Product_IDPA5-21627
Lot_IDUC2743613B
Host_organismrabbit
SourceThermo Fisher
Antibody_DCC_IDENCAB901VHL
Target Info
GeneNamePRPF39
Phosphate
Target_namePRPF39-human

Validation Images
Cell linePrimary validationSecondary validation
K562 ThermoFisher_PA5-21627_UC2743613B_PRPF39.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the PRPF39 specific antibody, PA5-21627. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PRPF39-specific antibody, PA5-21627. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the PRPF39 specific antibody, PA5-21627. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PRPF39-specific antibody, PA5-21627. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

PRPF39-K562-CRISPR-PA5-21627.png<br>Caption: Western blot following CRISPR against PRPF39 in K562 whole cell lysate using PRPF39 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PRPF39. PRPF39 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against PRPF39 in K562 whole cell lysate using PRPF39 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PRPF39. PRPF39 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGKcLV44-45

Status: Submitting

Lab: Graveley Lab