AKAP8L antibody: GTX115831


Antibody Info
Product_IDGTX115831
Lot_ID40296
Host_organismrabbit
SourceGeneTex
Antibody_DCC_IDENCAB936VHC
Target Info
GeneNameAKAP8L
Phosphate
Target_nameAKAP8L-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 AKAP8L-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against AKAP8L in HepG2 whole cell lysate using AKAP8L specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8L.AKAP8L protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against AKAP8L in HepG2 whole cell lysate using AKAP8L specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8L.AKAP8L protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV31-9

Status: Released

Lab: Graveley Lab

K562 k562_genetex_GTX115831_40296_AKAP8L.jpg<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using AKAP8L specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal AKAP8L antibody [N1N3] (lanes under 'AKAP8L').

Caption: IP-Western Blot analysis of K562 whole cell lysate using AKAP8L specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal AKAP8L antibody [N1N3] (lanes under 'AKAP8L').

Method: immunoprecipitation

Releated Sample: eCLIP:425

Status: Released

Lab: Yeo Lab

 AKAP8L-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against AKAP8L in K562 whole cell lysate using AKAP8L specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8L.AKAP8L protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against AKAP8L in K562 whole cell lysate using AKAP8L specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8L.AKAP8L protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV32-9

Status: Released

Lab: Graveley Lab