PARN antibody: GTX115020


Antibody Info
Product_IDGTX115020
Lot_ID40884
Host_organismrabbit
SourceGeneTex
Antibody_DCC_IDENCAB392QEW
Target Info
GeneNamePARN
Phosphate
Target_namePARN-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 PARN_Secondary_Western.png<br>Caption: Western blot following shRNA against PARN in K562 and HepG2 whole cell lysate using PARN specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PARN. Lanes 5-8 follow the same pattern, but in HepG2. PARN protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against PARN in K562 and HepG2 whole cell lysate using PARN specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PARN. Lanes 5-8 follow the same pattern, but in HepG2. PARN protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV24-43

Status: Released

Lab: Graveley Lab

K562 K562_genetex_GTX115020_40884_PARN.jpg<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PARN specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-PARN antibody (lanes under 'PARN').

Caption: IP-Western Blot analysis of K562 whole cell lysate using PARN specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-PARN antibody (lanes under 'PARN').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

PARN_Secondary_Western.png<br>Caption: Western blot following shRNA against PARN in K562 and HepG2 whole cell lysate using PARN specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PARN. Lanes 5-8 follow the same pattern, but in HepG2. PARN protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against PARN in K562 and HepG2 whole cell lysate using PARN specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PARN. Lanes 5-8 follow the same pattern, but in HepG2. PARN protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV24-43

Status: Released

Lab: Graveley Lab