GTF2F1 antibody: GTX114455


Antibody Info
Product_IDGTX114455
Lot_ID40723
Host_organismrabbit
SourceGeneTex
Antibody_DCC_IDENCAB108WPN
Target Info
GeneNameGTF2F1
Phosphate
Target_nameGTF2F1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Genetex_GTX114455_40723_GTF2F1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using GTF2F1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GTF2F1 antibody (lanes under 'GTF2F1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using GTF2F1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GTF2F1 antibody (lanes under 'GTF2F1').

Method: immunoprecipitation

Releated Sample: eCLIP:384

Status: Released

Lab: Yeo Lab

GTF2F1_Secondary_Western.png<br>Caption: Western blot following shRNA against GTF2F1 in K562 and HepG2 whole cell lysate using GTF2F1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GTF2F1. Lanes 5-8 follow the same pattern, but in HepG2. GTF2F1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against GTF2F1 in K562 and HepG2 whole cell lysate using GTF2F1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GTF2F1. Lanes 5-8 follow the same pattern, but in HepG2. GTF2F1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV13_39

Status: Released

Lab: Graveley Lab

K562 K562_Genetex_GTX114455_40723_GTF2F1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using GTF2F1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GTF2F1 antibody (lanes under 'GTF2F1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using GTF2F1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GTF2F1 antibody (lanes under 'GTF2F1').

Method: immunoprecipitation

Releated Sample: eCLIP:342

Status: Released

Lab: Yeo Lab

GTF2F1_Secondary_Western.png<br>Caption: Western blot following shRNA against GTF2F1 in K562 and HepG2 whole cell lysate using GTF2F1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GTF2F1. Lanes 5-8 follow the same pattern, but in HepG2. GTF2F1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against GTF2F1 in K562 and HepG2 whole cell lysate using GTF2F1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GTF2F1. Lanes 5-8 follow the same pattern, but in HepG2. GTF2F1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV13_39

Status: Released

Lab: Graveley Lab