Antibodies of GTF2F1


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
GTF2F1Product_ID: GTX114455
Lot_ID: 40723
Source: GeneTex
Target Name: GTF2F1-human		 HepG2_Genetex_GTX114455_40723_GTF2F1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using GTF2F1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GTF2F1 antibody (lanes under 'GTF2F1').
 GTF2F1_Secondary_Western.png<br>Caption: Western blot following shRNA against GTF2F1 in K562 and HepG2 whole cell lysate using GTF2F1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GTF2F1. Lanes 5-8 follow the same pattern, but in HepG2. GTF2F1 protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_Genetex_GTX114455_40723_GTF2F1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using GTF2F1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GTF2F1 antibody (lanes under 'GTF2F1').
 GTF2F1_Secondary_Western.png<br>Caption: Western blot following shRNA against GTF2F1 in K562 and HepG2 whole cell lysate using GTF2F1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GTF2F1. Lanes 5-8 follow the same pattern, but in HepG2. GTF2F1 protein appears as the green band, Tubulin serves as a control and appears in red.
GTF2F1Product_ID: PA5-30050
Lot_ID: TL2692997A
Source: Thermo Fisher
Target Name: GTF2F1-human		 GTF2F1-HEPG2-PA5-30050.png<br>Caption: Western blot following shRNA against GTF2F1 in HepG2 whole cell lysate using GTF2F1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against GTF2F1. GTF2F1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_PA5-30050_TL2692997A_GTF2F1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the GTF2F1 specific antibody, PA5-30050. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the GTF2F1-specific antibody, PA5-30050. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.