UCHL5 antibody: A304-099A


Antibody Info
Product_IDA304-099A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB421XHI
Target Info
GeneNameUCHL5
Phosphate
Target_nameUCHL5-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A304-099A_1_UCHL5.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using UCHL5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UCHL5 antibody (lanes under 'UCHL5').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using UCHL5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UCHL5 antibody (lanes under 'UCHL5').

Method: immunoprecipitation

Releated Sample: eCLIP:629

Status: Released

Lab: Yeo Lab

UCHL5_Secondary_Western.png<br>Caption: Western blot following shRNA against UCHL5 in K562 and HepG2 whole cell lysate using UCHL5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against UCHL5. Lanes 5-7 follow the same pattern, but in HepG2. UCHL5 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against UCHL5 in K562 and HepG2 whole cell lysate using UCHL5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against UCHL5. Lanes 5-7 follow the same pattern, but in HepG2. UCHL5 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV19-59

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A304-099A_1_UCHL5.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using UCHL5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UCHL5 antibody (lanes under 'UCHL5').

Caption: IP-Western Blot analysis of K562 whole cell lysate using UCHL5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UCHL5 antibody (lanes under 'UCHL5').

Method: immunoprecipitation

Releated Sample: eCLIP:610

Status: Released

Lab: Yeo Lab

UCHL5_Secondary_Western.png<br>Caption: Western blot following shRNA against UCHL5 in K562 and HepG2 whole cell lysate using UCHL5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against UCHL5. Lanes 5-7 follow the same pattern, but in HepG2. UCHL5 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against UCHL5 in K562 and HepG2 whole cell lysate using UCHL5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against UCHL5. Lanes 5-7 follow the same pattern, but in HepG2. UCHL5 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV19-59

Status: Released

Lab: Graveley Lab