NKRF antibody: A304-016A


Antibody Info
Product_IDA304-016A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB893CIV
Target Info
GeneNameNKRF
Phosphate
Target_nameNKRF-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A304-016A_1_NKRF.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NKRF specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NKRF antibody (lanes under 'NKRF').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NKRF specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NKRF antibody (lanes under 'NKRF').

Method: immunoprecipitation

Releated Sample: eCLIP:546

Status: Released

Lab: Yeo Lab

NKRF-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against NKRF in HepG2 whole cell lysate using NKRF specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NKRF.NKRF protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against NKRF in HepG2 whole cell lysate using NKRF specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NKRF.NKRF protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV27-37

Status: Released

Lab: Graveley Lab

K562 NKRF-k562.png<br>Caption: Western blot following shRNA against NKRF in k562 whole cell lysate using NKRF specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NKRF. NKRF protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against NKRF in k562 whole cell lysate using NKRF specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NKRF. NKRF protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKLV36-39

Status: Released

Lab: Graveley Lab