PRPF8 antibody: A303-921A


Antibody Info
Product_IDA303-921A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB207WIC
Target Info
GeneNamePRPF8
Phosphate
Target_namePRPF8-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_303-921A_1_PRPF8.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PRPF8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF8 antibody (lanes under 'PRPF8').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PRPF8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF8 antibody (lanes under 'PRPF8').

Method: immunoprecipitation

Releated Sample: eCLIP:291

Status: Released

Lab: Yeo Lab

PRPF8_Secondary_Western.png<br>Caption: Western blot following shRNA against PRPF8 in HepG2 whole cell lysate using PRPF8 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PRPF8. PRPF8 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against PRPF8 in HepG2 whole cell lysate using PRPF8 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PRPF8. PRPF8 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV17-23

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-921A_1_PRPF8.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PRPF8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF8 antibody (lanes under 'PRPF8').

Caption: IP-Western Blot analysis of K562 whole cell lysate using PRPF8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF8 antibody (lanes under 'PRPF8').

Method: immunoprecipitation

Releated Sample: eCLIP:278

Status: Released

Lab: Yeo Lab

PRPF8-K562-CRISPR.png<br>Caption: Western blot following CRISPR against PRPF8 in K562 whole cell lysate using PRPF8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PRPF8.PRPF8 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following CRISPR against PRPF8 in K562 whole cell lysate using PRPF8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PRPF8.PRPF8 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKcLV08-51

Status: Released

Lab: Graveley Lab