NSUN2 antibody: A303-912A


Antibody Info
Product_IDA303-912A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB643VMO
Target Info
GeneNameNSUN2
Phosphate
Target_nameNSUN2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 NSUN2-HEPG2.png<br>Caption: Western blot following shRNA against NSUN2 in HepG2 whole cell lysate using NSUN2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NSUN2.NSUN2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against NSUN2 in HepG2 whole cell lysate using NSUN2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NSUN2.NSUN2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV35-29

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-912A_1_NSUN2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using NSUN2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NSUN2 antibody (lanes under 'NSUN2').

Caption: IP-Western Blot analysis of K562 whole cell lysate using NSUN2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NSUN2 antibody (lanes under 'NSUN2').

Method: immunoprecipitation

Releated Sample: eCLIP:452

Status: Released

Lab: Yeo Lab

NSUN2-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against NSUN2 in K562 whole cell lysate using NSUN2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NSUN2. NSUN2 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against NSUN2 in K562 whole cell lysate using NSUN2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NSUN2. NSUN2 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV34-25

Status: Released

Lab: Graveley Lab