Antibodies of NSUN2


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NSUN2Product_ID: 711068
Lot_ID: SA245649
Source: Thermo Fisher
Target Name: NSUN2-human		 ThermoFisher_711068_SA245649_NSUN2_HepG2.png<br>Caption: IP-WB analysis of HepG2 whole cell lysate using the NSUN2 specific antibody, 711068. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment using a normal IgG antibody, respectively. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the NSUN2-specific antibody, 711068. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ThermoFisher_711068_SA245649_NSUN2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the NSUN2 specific antibody, 711068. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the NSUN2-specific antibody, 711068. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NSUN2-K562.png<br>Caption: Western blot following shRNA against NSUN2 in K562 whole cell lysate using NSUN2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against NSUN2. NSUN2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
NSUN2Product_ID: A303-912A
Lot_ID: 1
Source: Bethyl Labs
Target Name: NSUN2-human		 NSUN2-HEPG2.png<br>Caption: Western blot following shRNA against NSUN2 in HepG2 whole cell lysate using NSUN2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NSUN2.NSUN2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 K562_Bethyl_A303-912A_1_NSUN2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using NSUN2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NSUN2 antibody (lanes under 'NSUN2').
 NSUN2-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against NSUN2 in K562 whole cell lysate using NSUN2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NSUN2. NSUN2 protein appears as the green band, Tubulin serves as a control and appears in red.