FXR2 antibody: A303-894A


Antibody Info
Product_IDA303-894A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB069PGU
Target Info
GeneNameFXR2
Phosphate
Target_nameFXR2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A303-894A_1_FXR2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FXR2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FXR2 antibody (lanes under 'FXR2').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FXR2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FXR2 antibody (lanes under 'FXR2').

Comments: The placement of the arrow is incorrect, should be around the 85-90kDa mark as the band is running a bit high in lane 4.

Method: immunoprecipitation

Releated Sample: eCLIP:4098

Status: Released

Lab: Yeo Lab

FXR2-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against FXR2 in HepG2 whole cell lysate using FXR2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FXR2. FXR2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against FXR2 in HepG2 whole cell lysate using FXR2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FXR2. FXR2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHcLV05-17

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-894A_1_FXR2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using FXR2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FXR2 antibody (lanes under 'FXR2').

Caption: IP-Western Blot analysis of K562 whole cell lysate using FXR2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FXR2 antibody (lanes under 'FXR2').

Method: immunoprecipitation

Releated Sample: eCLIP:237, eCLIP:4002

Status: Released

Lab: Yeo Lab

FXR2-k562-CRISPR.png<br>Caption: Western blot following CRISPR against FXR2 in K562 whole cell lysate using FXR2 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FXR2. FXR2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against FXR2 in K562 whole cell lysate using FXR2 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FXR2. FXR2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKcLV04-13

Status: Released

Lab: Graveley Lab