U2AF2 antibody: A303-665A


Antibody Info
Product_IDA303-665A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB511TCT
Target Info
GeneNameU2AF2
Phosphate
Target_nameU2AF2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A303-665A_1_U2AF2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using U2AF2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-U2AF2 antibody (lanes under 'U2AF2').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using U2AF2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-U2AF2 antibody (lanes under 'U2AF2').

Method: immunoprecipitation

Releated Sample: eCLIP:272

Status: Released

Lab: Yeo Lab

U2AF2-LV26-HepG2.png<br>Caption: Western blot following shRNA against U2AF2 in HepG2 whole cell lysate using U2AF2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against U2AF2.U2AF2 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against U2AF2 in HepG2 whole cell lysate using U2AF2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against U2AF2.U2AF2 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGHLV26-63

Status: Released

Lab: Graveley Lab

U2AF2_Secondary_Western.png<br>Caption: Western blot following shRNA against U2AF2 in K562 and HepG2 whole cell lysate using U2AF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against U2AF2. Lanes 5-8 follow the same pattern, but in HepG2. U2AF2 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against U2AF2 in K562 and HepG2 whole cell lysate using U2AF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against U2AF2. Lanes 5-8 follow the same pattern, but in HepG2. U2AF2 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV08-63

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-665A_1_U2AF2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using U2AF2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-U2AF2 antibody (lanes under 'U2AF2').

Caption: IP-Western Blot analysis of K562 whole cell lysate using U2AF2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-U2AF2 antibody (lanes under 'U2AF2').

Method: immunoprecipitation

Releated Sample: eCLIP:242

Status: Released

Lab: Yeo Lab

U2AF2_Secondary_Western.png<br>Caption: Western blot following shRNA against U2AF2 in K562 and HepG2 whole cell lysate using U2AF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against U2AF2. Lanes 5-8 follow the same pattern, but in HepG2. U2AF2 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against U2AF2 in K562 and HepG2 whole cell lysate using U2AF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against U2AF2. Lanes 5-8 follow the same pattern, but in HepG2. U2AF2 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV08-63

Status: Released

Lab: Graveley Lab