PKM antibody: A303-660A


Antibody Info
Product_IDA303-660A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB905ZIN
Target Info
GeneNamePKM
Phosphate
Target_namePKM-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A303-660A_1_PKM2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PKM2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PKM2 antibody (lanes under 'PKM2').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PKM2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PKM2 antibody (lanes under 'PKM2').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

pkm2_Secondary_Western.png<br>Caption: Western blot following shRNA against pkm2 in HepG2 whole cell lysate using pkm2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against pkm2. pkm2 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against pkm2 in HepG2 whole cell lysate using pkm2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against pkm2. pkm2 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV16-39

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-660A_1_PKM.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PKM specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PKM antibody (lanes under 'PKM').

Caption: IP-Western Blot analysis of K562 whole cell lysate using PKM specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PKM antibody (lanes under 'PKM').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

PKM2-k562.png<br>Caption: Western blot following shRNA against PKM2 in k562 whole cell lysate using PKM2 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PKM2.PKM2 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against PKM2 in k562 whole cell lysate using PKM2 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PKM2.PKM2 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV21-41

Status: Released

Lab: Graveley Lab