Antibodies of PKM


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
PKMProduct_ID: A303-660A
Lot_ID: 1
Source: Bethyl Labs
Target Name: PKM-human		 HepG2_Bethyl_A303-660A_1_PKM2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PKM2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PKM2 antibody (lanes under 'PKM2').
 pkm2_Secondary_Western.png<br>Caption: Western blot following shRNA against pkm2 in HepG2 whole cell lysate using pkm2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against pkm2. pkm2 protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_Bethyl_A303-660A_1_PKM.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PKM specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PKM antibody (lanes under 'PKM').
 PKM2-k562.png<br>Caption: Western blot following shRNA against PKM2 in k562 whole cell lysate using PKM2 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PKM2.PKM2 protein appears as the green band, Tubulin serves as a control and appears in red.
PKMProduct_ID: 4053S
Lot_ID: 6
Source: Cell Signaling Technology
Target Name: PKM-human		 PKM-HEPG2-CRISPR-4053S.png<br>Caption: Western blot following CRISPR against PKM in HepG2 whole cell lysate using PKM specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PKM. PKM protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_4053S_6_PKM2.png<br>Caption: IP-WB analysis of 4053S whole cell lysate using the PKM2 specific antibody, 4053S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PKM2-specific antibody, 4053S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 PKM-K562-CRISPR-4053S.png<br>Caption: Western blot following CRISPR against PKM in K562 whole cell lysate using PKM specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PKM. PKM protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.