AARS antibody: A303-475A


Antibody Info
Product_IDA303-475A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB346SAT
Target Info
GeneNameAARS
Phosphate
Target_nameAARS-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A303-475A_1_AARS.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AARS specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AARS antibody (lanes under 'AARS').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AARS specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AARS antibody (lanes under 'AARS').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

AARS_Secondary_Western.png<br>Caption: Western blot following shRNA against AARS in K562 or HepG2 whole cell lysate using AARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against AARS. Lanes 5-7 follow the same pattern, but in HepG2. AARS protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against AARS in K562 or HepG2 whole cell lysate using AARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against AARS. Lanes 5-7 follow the same pattern, but in HepG2. AARS protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV21-3

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-475A_1_AARS.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using AARS specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AARS antibody (lanes under 'AARS').

Caption: IP-Western Blot analysis of K562 whole cell lysate using AARS specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AARS antibody (lanes under 'AARS').

Method: immunoprecipitation

Releated Sample: eCLIP:624

Status: Released

Lab: Yeo Lab

AARS_Secondary_Western.png<br>Caption: Western blot following shRNA against AARS in K562 or HepG2 whole cell lysate using AARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against AARS. Lanes 5-7 follow the same pattern, but in HepG2. AARS protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against AARS in K562 or HepG2 whole cell lysate using AARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against AARS. Lanes 5-7 follow the same pattern, but in HepG2. AARS protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV21-3

Status: Released

Lab: Graveley Lab