| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using ZNF800 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ZNF800 antibody (lanes under 'ZNF800'). Comments: The marked band is similar in size to that of the one seen in K562 that was analyzed by mass spec. We believe it is the protein of interest. Method: immunoprecipitation Releated Sample: eCLIP:743 Status: Released Lab: Yeo Lab | |
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| K562 | 
Caption: Representative image of immunoprecipitation performed on whole cell extracts from the K562 cell line using the ZNF800-specific antibody A303-283A. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using ZNF800 antibody. Lane 4: Immunoprecipitated material using ZNF800 antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 75 kDa. Comments: The main immunoreactive band is higher than expected but we show by mass spectrometry that it is detecting the protein of interest. Method: immunoprecipitation Releated Sample: eCLIP:737 Status: Released Lab: Yeo Lab | 
Caption: Western blot following CRISPR against ZNF800 in K562 whole cell lysate using ZNF800 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ZNF800. ZNF800 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow. Releated Sample: BGKcLV06-47 Status: Released Lab: Graveley Lab 
Caption: IP followed by mass spectrometry. Protein was immunoprecipitated from K562 whole cell lysates using the antibody A303-283A, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility. Status: Released Lab: Yeo Lab |
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