SF1 antibody: A303-214A


Antibody Info
Product_IDA303-214A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB525DSF
Target Info
GeneNameSF1
Phosphate
Target_nameSF1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 SF1-HEPG2-A303-214A.png<br>Caption: Western blot following shRNA against SF1 in HepG2 whole cell lysate using SF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SF1. SF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following shRNA against SF1 in HepG2 whole cell lysate using SF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against SF1. SF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHLV26-51

Status: Experimented

Lab: Graveley Lab

K562 Bethyl_A303-214A_1_SF1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SF1 specific antibody, A303-214A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SF1-specific antibody, A303-214A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the SF1 specific antibody, A303-214A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SF1-specific antibody, A303-214A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab