Antibodies of SF1


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SF1Product_ID: A303-213A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SF1-human		 Bethyl_A303-213A_1_SF1.png<br>Caption: Immunoprecipitation lane (3) exhibits a large deviation from the expected protein size, which we believe is due to performing the characterization using the Jess Western Blotting system. This system has a documented tendency for some proteins to run differently than with more traditional methods due to their compositions and interactions with the electrophoretic components. The image also closely resembles the example image provided by the vendor but secondary validation will be needed for verification.
SF1Product_ID: A303-214A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SF1-human		 Bethyl_A303-214A_1_SF1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SF1 specific antibody, A303-214A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SF1-specific antibody, A303-214A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
SF1Product_ID: GTX104540
Lot_ID: 40002
Source: GeneTex
Target Name: SF1-human		 SF1_Secondary_Western.png<br>Caption: Western blot following shRNA against SF1 in K562 and HepG2 whole cell lysate using SF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF1. Lanes 5-8 follow the same pattern, but in HepG2. SF1 protein appears as the green band, Tubulin serves as a control and appears in red.
 SF1_Secondary_Western.png<br>Caption: Western blot following shRNA against SF1 in K562 and HepG2 whole cell lysate using SF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF1. Lanes 5-8 follow the same pattern, but in HepG2. SF1 protein appears as the green band, Tubulin serves as a control and appears in red.