FUBP3 antibody: A303-012A


Antibody Info
Product_IDA303-012A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB964AJF
Target Info
GeneNameFUBP3
Phosphate
Target_nameFUBP3-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A303-012A_1_FUBP3.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FUBP3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FUBP3 antibody (lanes under 'FUBP3').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FUBP3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FUBP3 antibody (lanes under 'FUBP3').

Method: immunoprecipitation

Releated Sample: eCLIP:571

Status: Released

Lab: Yeo Lab

FUBP3_Secondary_Western.png<br>Caption: Western blot following shRNA against FUBP3 in HepG2 whole cell lysate using FUBP3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP3. FUBP3 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against FUBP3 in HepG2 whole cell lysate using FUBP3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP3. FUBP3 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV12-27

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-012A_1_FUBP3.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using FUBP3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FUBP3 antibody (lanes under 'FUBP3').

Caption: IP-Western Blot analysis of K562 whole cell lysate using FUBP3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FUBP3 antibody (lanes under 'FUBP3').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

FUBP3-K562-CRISPR.png<br>Caption: Western blot following CRISPR against FUBP3 in K562 whole cell lysate using FUBP3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FUBP3.FUBP3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against FUBP3 in K562 whole cell lysate using FUBP3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against FUBP3.FUBP3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKcLV08-35

Status: Released

Lab: Graveley Lab