XRCC5 antibody: A302-627A


Antibody Info
Product_IDA302-627A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB457QLP
Target Info
GeneNameXRCC5
Phosphate
Target_nameXRCC5-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HEPG2_bethyl_A302-627A_1_XRCC5.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using XRCC5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-XRCC5 antibody (lanes under 'XRCC5').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using XRCC5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-XRCC5 antibody (lanes under 'XRCC5').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

XRCC5-HEPG2.png<br>Caption: Western blot following shRNA against XRCC5 in HepG2 whole cell lysate using XRCC5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against XRCC5. XRCC5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against XRCC5 in HepG2 whole cell lysate using XRCC5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against XRCC5. XRCC5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV14-67

Status: Released

Lab: Graveley Lab

K562 K562_bethyl_A302-627A_1_XRCC5.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using XRCC5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-XRCC5 antibody (lanes under 'XRCC5').

Caption: IP-Western Blot analysis of K562 whole cell lysate using XRCC5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-XRCC5 antibody (lanes under 'XRCC5').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

XRCC5-K562-CRISPR.png<br>Caption: Western blot following CRISPR against XRCC5 in K562 whole cell lysate using XRCC5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against XRCC5. XRCC5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against XRCC5 in K562 whole cell lysate using XRCC5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against XRCC5. XRCC5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKcLV11-111

Status: Released

Lab: Graveley Lab