Antibodies of XRCC5


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
XRCC5Product_ID: A302-627A
Lot_ID: 1
Source: Bethyl Labs
Target Name: XRCC5-human
HEPG2_bethyl_A302-627A_1_XRCC5.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using XRCC5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-XRCC5 antibody (lanes under 'XRCC5').
XRCC5-HEPG2.png<br>Caption: Western blot following shRNA against XRCC5 in HepG2 whole cell lysate using XRCC5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against XRCC5. XRCC5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
K562_bethyl_A302-627A_1_XRCC5.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using XRCC5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-XRCC5 antibody (lanes under 'XRCC5').
XRCC5-K562-CRISPR.png<br>Caption: Western blot following CRISPR against XRCC5 in K562 whole cell lysate using XRCC5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against XRCC5. XRCC5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
XRCC5Product_ID: 201004
Lot_ID: DD0729
Source: Zen Bioscience
Target Name: XRCC5-human
XRCC5_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using XRCC5 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-XRCC5 antibody (lanes under 'anti-XRCC5').
XRCC5-HEPG2_FU's.png<br>Caption: Western blot following shRNA against XRCC5 in HepG2 whole cell lysate using XRCC5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against XRCC5.XRCC5 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
XRCC5-HEPG2_FU%27s.png<br>Caption: Western blot following shRNA against XRCC5 in HepG2 whole cell lysate using XRCC5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against XRCC5.XRCC5 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
XRCC5_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using XRCC5 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-XRCC5 antibody (lanes under 'anti-XRCC5').
XRCC5Product_ID: 2180S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: XRCC5-human
CST_2180S_1_XRCC5.png<br>Caption: IP-WB analysis of 2180S whole cell lysate using the XRCC5 specific antibody, 2180S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the XRCC5-specific antibody, 2180S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
XRCC5-K562-CRISPR-2180S.png<br>Caption: Western blot following CRISPR against XRCC5 in K562 whole cell lysate using XRCC5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against XRCC5. XRCC5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.