SF3A3 antibody: A302-506A


Antibody Info
Product_IDA302-506A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB757ZZH
Target Info
GeneNameSF3A3
Phosphate
Target_nameSF3A3-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A302-506A_1_SF3A3.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SF3A3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3A3 antibody (lanes under 'SF3A3').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SF3A3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3A3 antibody (lanes under 'SF3A3').

Method: immunoprecipitation

Releated Sample: eCLIP:393

Status: Released

Lab: Yeo Lab

SF3A3-HEPG2.png<br>Caption: Western blot following shRNA against SF3A3 in HepG2 whole cell lysate using SF3A3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3A3.SF3A3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against SF3A3 in HepG2 whole cell lysate using SF3A3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3A3.SF3A3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV33-29

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A302-506A_1_SF3A3.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using SF3A3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3A3 antibody (lanes under 'SF3A3').

Caption: IP-Western Blot analysis of K562 whole cell lysate using SF3A3 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3A3 antibody (lanes under 'SF3A3').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

SF3A3-K562.png<br>Caption: Western blot following shRNA against SF3A3 in K562 whole cell lysate using SF3A3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3A3. SF3A3 serves as a control and appears in red arrow.GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against SF3A3 in K562 whole cell lysate using SF3A3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3A3. SF3A3 serves as a control and appears in red arrow.GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKLV34-37

Status: Released

Lab: Graveley Lab